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bs 3576r  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology bs 3576r
    Bs 3576r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bs+3576r/EGFR/pm35734041-72-47-52
    Average 96 stars, based on 2221 article reviews
    bs 3576r - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunostaining:

    Article Title: Analysis of the epidermal growth factor receptor/phosphoinositide-dependent protein kinase-1 axis in tumor of the external auditory canal in response to epidermal growth factor stimulation.
    Article Snippet: All analyses were performed using a statistical software package (JMP version 13; SAS Institute Japan, Tokyo, Japan). .. TABLE 1 Antibodies used for immunostaining Antigen, clone name, or immunogen Manufacturer, species, catalog no. Dilution used Type FLAG, M2 Sigma-Aldrich, St. Louis, MO, mouse, F1804 1:500 Primary Ki67, mouse KI67 aa.1850-1950 Novus Biologicals, Centennial, CO, rabbit, NB110-89717 1:400 Primary EGF, 90-140/208 Bioss Antibodies, Woburn, MA, rabbit, bs-3576R 1:200 Primary EGFR, A-10 Santa Cruz Biotechnology, Dallas, TX, mouse, sc- 373746 1:50 Primary EGFR, EP38Y Abcam, Cambridge, UK, rabbit, ab52894 1:100 Primary PDPK1, E3 Santa Cruz Biotechnology, Dallas, TX, mouse, sc- 17765 1:100 Primary PDPK1, EP569Y Abcam, Cambridge, UK, rabbit, ab52893 1:150 Primary Cleaved caspase-3, Asp175 Cell signaling Technology, Danvers, MA, rabbit, #9661 1:200 Primary HRP-goat anti-mouse IgG Abcam, Cambridge, UK, goat, ab6789 1:100 Secondary HRP-goat anti-rabbit IgG Abcam, Cambridge, UK, goat, ab6721 1:100 Secondary Alexa Fluor 488-goat anti-mouse IgG Thermo Fisher Scientific, Hudson, NH, goat, A-11008 1:500 Secondary Alexa Fluor 555-goat anti-rabbit IgG Thermo Fisher Scientific, Hudson, NH, goat, A-21422 1:500 Secondary 732 AKIYAMA ET AL. 23788038, 2022, 3, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/lio2.785, W iley O nline L ibrary on [11/06/2024]. ..



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    Antibodies used for immunostaining

    Journal: Laryngoscope Investigative Otolaryngology

    Article Title: Analysis of the epidermal growth factor receptor/phosphoinositide‐dependent protein kinase‐1 axis in tumor of the external auditory canal in response to epidermal growth factor stimulation

    doi: 10.1002/lio2.785

    Figure Lengend Snippet: Antibodies used for immunostaining

    Article Snippet: EGF, 90‐140/208 , Bioss Antibodies, Woburn, MA, rabbit, bs‐3576R , 1:200 , Primary.

    Techniques:

    Western blotting demonstrates HB-EGF processing during OM. (A) Representative blots for rat MEs are shown. The processed form, sHB-EGF, was distinguished from proHB-EGF by its lower molecular weight, (B) Relative band intensity quantification confirms that sHB-EGF increases to a maximum 2 days after administration of 500 μg of Poly(I:C), then progressively decreases. Saline control mucosa at 2 days shows a low sHB-EGF level. N refers to number of samples. *p < 0.05, **p < 0.01.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: HB-EGF Plays a Pivotal Role in Mucosal Hyperplasia During Otitis Media Induced by a Viral Analog

    doi: 10.3389/fcimb.2022.823714

    Figure Lengend Snippet: Western blotting demonstrates HB-EGF processing during OM. (A) Representative blots for rat MEs are shown. The processed form, sHB-EGF, was distinguished from proHB-EGF by its lower molecular weight, (B) Relative band intensity quantification confirms that sHB-EGF increases to a maximum 2 days after administration of 500 μg of Poly(I:C), then progressively decreases. Saline control mucosa at 2 days shows a low sHB-EGF level. N refers to number of samples. *p < 0.05, **p < 0.01.

    Article Snippet: After quenching endogenous peroxidases, sections were incubated with anti-HB-EGF antibody (rabbit polyclonal, 1:200, Bioss) that recognizes both forms of the factor for 24 hours.

    Techniques: Western Blot, Molecular Weight

    An HB-EGF inhibitor decreases mucosal thickness during OM. Poly(I:C)-induced hyperplasia of rat ME mucosa was inhibited by co-administration of CRM197. (A) Representative microphotographs are shown on day 2. Scale bar = 100 μm. (B) Mean thickness of saline ME mucosa versus 500 ug of Poly(I:C), without or with various levels of CRM197 on day 2. N refers to number of MEs. *p < 0.05; **p < 0.01.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: HB-EGF Plays a Pivotal Role in Mucosal Hyperplasia During Otitis Media Induced by a Viral Analog

    doi: 10.3389/fcimb.2022.823714

    Figure Lengend Snippet: An HB-EGF inhibitor decreases mucosal thickness during OM. Poly(I:C)-induced hyperplasia of rat ME mucosa was inhibited by co-administration of CRM197. (A) Representative microphotographs are shown on day 2. Scale bar = 100 μm. (B) Mean thickness of saline ME mucosa versus 500 ug of Poly(I:C), without or with various levels of CRM197 on day 2. N refers to number of MEs. *p < 0.05; **p < 0.01.

    Article Snippet: After quenching endogenous peroxidases, sections were incubated with anti-HB-EGF antibody (rabbit polyclonal, 1:200, Bioss) that recognizes both forms of the factor for 24 hours.

    Techniques:

    CRM197 does not inhibit HB-EGF expression or processing. (A) Representative microphotographs of immunostaining with a pan anti-HB-EGF antibody are shown. HB-EGF was expressed in rat ME mucosa in all 3 groups on day 2. (B) Representative Western blot sHB-EGF bands for rat ME, 2 days after saline injection or Poly(I:C) administration with or without CRM197. (C) Quantitative analysis showed that sHB-EGF was low in the saline samples but was increased by both Poly(I:C) and Poly(I:C) plus CRM197. N refers to number of samples. *p < 0.05.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: HB-EGF Plays a Pivotal Role in Mucosal Hyperplasia During Otitis Media Induced by a Viral Analog

    doi: 10.3389/fcimb.2022.823714

    Figure Lengend Snippet: CRM197 does not inhibit HB-EGF expression or processing. (A) Representative microphotographs of immunostaining with a pan anti-HB-EGF antibody are shown. HB-EGF was expressed in rat ME mucosa in all 3 groups on day 2. (B) Representative Western blot sHB-EGF bands for rat ME, 2 days after saline injection or Poly(I:C) administration with or without CRM197. (C) Quantitative analysis showed that sHB-EGF was low in the saline samples but was increased by both Poly(I:C) and Poly(I:C) plus CRM197. N refers to number of samples. *p < 0.05.

    Article Snippet: After quenching endogenous peroxidases, sections were incubated with anti-HB-EGF antibody (rabbit polyclonal, 1:200, Bioss) that recognizes both forms of the factor for 24 hours.

    Techniques: Expressing, Immunostaining, Western Blot, Injection